Southern+Blotting+and+DNA+Microarray

Denisse Perez Denisse Simonian

**__Southern Blotting and DNA Microarray__**
Invented by: Edwin Southern ||= ===What it is:=== It detects a specific gene on DNA. ||= ===How it works:=== First, scientists separate the DNA and put it on a sheet. Then, they apply Agarose gel on the sheet. Afterwards, they take a membrane and put it on top of the gel. Next, the gel transfers the DNA to the membrane. Then, they inject a probe that contains the complementary sequence of the gene that they are finding into the membrane. Finally, the probe finds the gene and makes it radioactively glow. ||= ===How we use it:=== Scientist use it to see if there is any kind of disorder in the gene. || Invented by: Pat Brown ||= It detects if a gene is active or not. It points out cancer. ||= First, it collects healthy and cancerous tissue samples. Then the messenger RNA (mRNA) is segregated from the samples. The mRNA is color-coded and makes a complementary DNA strand and it is put on the microarray. The base pairs on the DNA bind to the complementary bases on the array. When scanned with a laser, spots will either turn red, green, or yellow. Red = Expressed in cancer cells. Green = Repressed in cancer cells. Yellow = Neutral. Black = Inactive gene. ||= We use it to study cancer and it helps researchers come up with new and better treatments for cancer. We also use it to help us classify a type of cancer, depending on its location. It compares healthy cells to cancerous cells. ||
 * = ===Southern blotting:===
 * = ===DNA Microarray:===

http://www.youtube.com/watch?v=VNsThMNjKhM http://www.youtube.com/watch?v=vNXBz9cURFo&feature=related
 * DNA Microarray Video:**



http://www.dnatube.com/video/1512/Southern-blot
 * Southern Blotting Video:**